ChromoTek p53 C-term-Trap Agarose
p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.
Cat No : pta2
Synonyms
Validation Data Gallery
Product Information
p53-C-term-Trap Agarose is an affinity resin for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads and that binds to the C-terminus of p53.
| Description | Immunoprecipitation of p53 with p53-C-term-Trap Agarose.
• Immunoprecipitation of human and mice p53 isoforms alpha with p53-C-term-Trap • Pull-down of the human p53 proteome with complementary p53-N-term- and p53-C-term-Traps, e.g. p53 isoforms and variants • No heavy & light antibody chains in your downstream application • Short incubation time (60 min at +4°C) • Strong binding even under harsh washing conditions |
| Applications | IP, CoIP |
| Specificity/Target | p53 (isoform alpha); epitope 302-393 aa |
| Tested Reactivity | Human, Mouse |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Compatibility with mass spectrometry | The p53-C-term-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631379 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
| SDS |
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| p53-C-term-Trap Agarose SDS (PDF) |
| Protocol |
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| p53-C-term-Trap Agarose protocol (PDF) |
| Product flyer |
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| Mdm4- HdmX-Trap, p53-N-term-Trap & p53-C-term-Trap product flyer (PDF) |
Other Formats
| p53 C term-Trap Agarose kit |
Publications
| Application | Title |
|---|---|
Cell Death Differ The ubiquitin ligase LIN41/TRIM71 targets p53 to antagonize cell death and differentiation pathways during stem cell differentiation. | |
PLoS Pathog Novel modulators of p53-signaling encoded by unknown genes of emerging viruses. | |
J Virol The ORF45 protein of Kaposi Sarcoma-associated Herpesvirus (KSHV) is an inhibitor of p53 signaling during viral reactivation. | |