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NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (8)

Applications

FC, IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10745-1-AP

Print datasheet

Synonyms

NF Kappa B, NF κB, NFKB 3, NFKB p65, NFKB3, NF-κB, NF-κB3, p65, RELA, Transcription factor p65



经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, MCF-7 cells, K-562 cells, Hek-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human stomach tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®650 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

10745-1-AP targets NF-κB p65 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications chIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human
Cited Reactivityhuman, Bombyx mori, bovine, canine, chicken, fish, hamster, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199 种属同源性预测
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Synonyms NF Kappa B, NF κB, NFKB 3, NFKB p65, NFKB3, NF-κB, NF-κB3, p65, RELA, Transcription factor p65
Calculated molecular weight 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC011603
Gene symbol RELA
UniprotQ04206
Gene ID (NCBI) 5970
RRIDAB_2178878
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

实验方案

Product Specific Protocols
WB protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IHC protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IF protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IP protocol for NF-κB p65 antibody 10745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Blood

PMID: 32291445

Authors - Kunpeng Wu
humanWB

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang
mouseWB

Nat Commun

REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis.

Authors - Shuai Wang
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang
pigWB

Nucleic Acids Res

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Authors - Xin Zong
mouseWB, IF

Biomaterials

Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection.

Authors - Yingying Shi