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验证数据展示

  • WB analysis of Hek-293 cells using 10745-1-AP

    WB analysis of Hek-293 cells using 10745-1-AP

    WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

  • WB analysis using 10745-1-AP

    WB analysis using 10745-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10745-1-AP

    IHC staining of human breast cancer using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
  • IF Staining of HepG2 using 10745-1-AP

    IF Staining of HepG2 using 10745-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 10745-1-AP

    IP experiment of HeLa using 10745-1-AP

    IP result of anti-NF-κB p65 (IP:10745-1-AP, 4ug; Detection:10745-1-AP 1:6000) with HeLa cells lysate 1280 ug.

  • IHC staining of mouse colon using 10745-1-AP

    IHC staining of mouse colon using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
  • IHC staining of human colon using 10745-1-AP

    IHC staining of human colon using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with NF-κB p65/RELA antibody included
  • IF Staining of HT-1080 using 10745-1-AP

    IF Staining of HT-1080 using 10745-1-AP

    Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using NF-κB p65 antibody (10745-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10745-1-AP

产品说明书

引用文献(1544)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (9)

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

NF κB, p65, p65; RELA, RELA, NF Kappa B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, MCF-7 cells, K-562 cells, Hek-293 cells, HeLa cells, Raji cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human liver cancer tissue, human stomach tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, TNF alpha treated HT-1080 cells
Planning an IHC experiment? We recommend our IHCeasy NF-κB p65/RELA Ready-To-Use IHC Kit. NF-κB p65/RELA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10745-1-AP targets NF-κB p65 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, chIP, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, canine, monkey, chicken, bovine, hamster, goat, fish, bombyx mori, duck
免疫原 NF-κB p65 fusion protein Ag1199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-rel reticuloendotheliosis viral oncogene homolog A (avian)
别名 NF κB, p65, p65; RELA, RELA, NF Kappa B
计算分子量 65 kDa
观测分子量 65 kDa
GenBank蛋白编号BC011603
基因名称 NF-κB p65
Gene ID (NCBI) 5970
RRIDAB_2178878
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ04206
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

实验方案

Product Specific Protocols
WB protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IHC protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IF protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IP protocol for NF-κB p65 antibody 10745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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mouseWB

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mouseIF

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humanWB

Nat Microbiol

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humanWB

Adv Sci (Weinh)

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