Validation Data Gallery
Technical Specifications
| GeneID | 3593 |
| Species | Human |
| Expression | HEK293 |
| Activity | Inquire |
| Purity | > 95% |
| Endotoxin | < 0.1 EU/ug |
| Accession Number | P29460 |
| Molecular Mass | 37-50 kDa reduced, 37 to 47, 65 to 95, and 125 kDa non-reduced, monomer, glycosylated |
| Formulation | 1x PBS pH 7.4 |
| Species Reactivity | human |
Stability and Reconstitution
| Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
|---|---|---|---|
| Lyophilized | -20°C to -80°C | Until Expiry Date | |
| Lyophilized | Room Temperature | 2 weeks | |
| Reconstituted as per CofA | -20°C to -80°C | 6 months | |
| Reconstituted as per CofA | 4°C | 1 week | |
| Avoid repeated freeze-thaw cycles. | |||
| Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix. |
Background
IL-12p40, also known as IL-12B, is a subunit of both the IL-12 and IL-23 cytokines. It is not thought to be bioactive on its own, but it has been shown to regulate IL-12 activity in antagonistic manner by competitively binding with the IL-12 receptor. IL-12p40 also functions as a chemoattractant molecule for macrophage infiltration into tumor sites. There has additionally been evidence to suggest that IL-12p40 mediates a protective immune response against mycobacterial infection by serving as a necessary component for granuloma formation and promoting Th1 effector cell functions (PMID: 17126601, 10453037, 11739515).
Synonyms
Interleukin-12 subunit beta (IL-12B) Cytotoxic lymphocyte maturation factor (CLMF p40), IL-12 subunit p40, NK cell stimulatory factor chain 2 (NKSF2)