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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human OSM protein

Activity

0.1-1.5 ng/mL EC50

Specific Activity

3.07 x 107 IU/mg

Species Reactivity

human

Purity

>95%

Cat No : HZ-1030

Print datasheet

Synonyms

oncostatin M, OSM

Validation Data Gallery

  • Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human OSM (HZ-1030) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.5 ng/mL​.

    Recombinant human OSM (HZ-1030) stimulates dose-dependent proliferation of the TF-1 human erythroleukemic indicator cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.5 ng/mL​.

Technical Specifications

GeneID 5008
Species Human
Expression HEK293
Activity 0.1-1.5 ng/mL EC50
Specific Activity3.07 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP13725
Molecular Mass 30 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and Storage Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Oncostatin M (OSM) is a member of a cytokine family that includes a leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. OSM is a growth regulator which inhibits the proliferation of some tumor cell lines. It can stimulate proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF, and GM-CSF from endothelial cells. OSM is involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (PMID: 9133674; 29526757; 29363180; 21887386).

Synonyms

oncostatin M, OSM

Publications

SpeciesTitle
human

Oncotarget

CM363, a novel naphthoquinone derivative which acts as multikinase modulator and overcomes imatinib resistance in chronic myelogenous leukemia.

Authors - BorjaGuerra
View Article
human

Invest Ophthalmol Vis Sci

Role of Oncostatin M in the Pathogenesis of Vernal Keratoconjunctivitis: Focus on the Barrier Function of the Epithelium and Interleukin-33 Production by Fibroblasts

Authors - Ishin Ninomiya
View Article
human

Cancer Biomark

Interleukin-6 and oncostatin M are elevated in liver disease in conjunction with candidate hepatocellular carcinoma biomarker GP73.

Authors - HongyanLiang
View Article
human

Front Pharmacol

A Novel Naphthoquinone-Coumarin Hybrid That Inhibits BCR-ABL1-STAT5 Oncogenic Pathway and Reduces Survival in Imatinib-Resistant Chronic Myelogenous Leukemia Cells.

Authors - Patricia Martín-Rodríguez
View Article