Various lysates were subjected to SDS PAGE followed by western blot with 14961-1-AP (RAD51 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WB result of RAD51 antibody (14961-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD51 transfected NIH/3T3 cells.

HeLa cells were subjected to SDS PAGE followed by western blot with 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP250/CNAP1 antibody (14498-1-AP) at dilution of 1:100 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L), SCLT1 antibody (14875-1-AP, Magenta), CoraLite®488 ARL13B antibody (CL488-17711, green).

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14498-1-AP (CEP250/CNAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14498-1-AP (CEP250/CNAP1 antibody at dilution of 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14498-1-AP (CEP250/CNAP1 antibody at dilution of 1:200 (under 40x lens).

IP Result of anti-CEP250/CNAP1 (IP:14498-1-AP, 5ug; Detection:14498-1-AP 1:500) with HeLa cells lysate 1200ug.

Various lysates were subjected to SDS PAGE followed by western blot with 10305-1-AP (PLK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10305-1-AP (PLK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human PLK1 (10305-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

K-562 cells were subjected to SDS PAGE followed by western blot with 10743-1-AP (Centromere protein R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10743-1-AP (Centromere protein R antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (-20℃ Ethanol) fixed MDCK cells using 22271-1-AP (Pericentrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CEP250,C-NAP1 fusion protein Ag5925 SDS-PAGE

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.