Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig and More (2)

Applications

FC, IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2B5A7

Cat No : 66305-1-Ig

Print datasheet

Synonyms

CMD1W, Metavinculin, MVCL, VCL, Vinculin



经过测试的应用

Positive WB detected inHepG2 cells, pig heart tissue, U-251 cells, LNCaP cells, U-87 MG cells, C6 cells, HEK-293 cells, HeLa cells, HuH-7 cells, L02 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66305-1-Ig targets Vinculin in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, WB
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, zebrafish, hamster, pig
Immunogen Vinculin fusion protein Ag24946 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name vinculin
Synonyms CMD1W, Metavinculin, MVCL, VCL, Vinculin
Calculated Molecular Weight 1133 aa, 124 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC039174
Gene Symbol VCL
Gene ID (NCBI) 7414
RRIDAB_2810300
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

实验方案

Product Specific Protocols
WB protocol for Vinculin antibody 66305-1-IgDownload protocol
IHC protocol for Vinculin antibody 66305-1-IgDownload protocol
IF protocol for Vinculin antibody 66305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Crit Care

Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein.

Authors - Lingbing Zhang
humanWB

Nat Commun

Modular assembly of the principal microtubule nucleator γ-TuRC.

Authors - Martin Würtz
mouseIF

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
humanWB

Redox Biol

SPTBN2 suppresses ferroptosis in NSCLC cells by facilitating SLC7A11 membrane trafficking and localization

Authors - Jun Deng
humanWB

Cell Death Differ

Loss of RBMS1 promotes anti-tumor immunity through enabling PD-L1 checkpoint blockade in triple-negative breast cancer.

Authors - Jinrui Zhang
humanWB

Cell Death Differ

Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.

Authors - Weiwei Cui