验证数据展示
经过测试的应用
| Positive WB detected in | HT-29 cells, Caco-2 cells, human placenta tissue, NCCIT cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67439-1-Ig targets SOX9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, rabbit, canine |
| 免疫原 | SOX9 fusion protein Ag28620 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SRY (sex determining region Y)-box 9 |
| 别名 | Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1 |
| 计算分子量 | 509 aa, 56 kDa |
| 观测分子量 | 56 kDa |
| GenBank蛋白编号 | BC007951 |
| 基因名称 | SOX9 |
| Gene ID (NCBI) | 6662 |
| RRID | AB_2882675 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P48436 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SOX9 antibody 67439-1-Ig | Download protocol |
| FC protocol for SOX9 antibody 67439-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology | ||
Theranostics Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI | ||
Mater Today Bio Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction | ||
J Nanobiotechnology Dual-dynamic-bond cross-linked injectable hydrogel of multifunction for intervertebral disc degeneration therapy | ||
Free Radic Biol Med Iron overload promotes intervertebral disc degeneration via inducing oxidative stress and ferroptosis in endplate chondrocytes | ||
EBioMedicine Decreased miR-214-3p activates NF-κB pathway and aggravates osteoarthritis progression. |
