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验证数据展示

  • FC experiment of HeLa using CL488-67439

    FC experiment of HeLa using CL488-67439

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SOX9 (CL488-67439, Clone:1H9A6) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SOX9 Monoclonal antibody

SOX9 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67439

产品说明书

CloneNo. 1H9A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

FC (Intra)

别名

Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67439-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67439 targets SOX9 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 SOX9 fusion protein Ag28620 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 9
别名 Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1
计算分子量 509 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC007951
基因名称 SOX9
Gene ID (NCBI) 6662
RRIDAB_3084349
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP48436
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SOX9 antibody CL488-67439Download protocol
Standard Protocols
Click here to view our Standard Protocols