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  • Featured Product
  • KD/KO Validated

SOX9 Monoclonal antibody, PBS Only

SOX9 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1H9A6

Cat no : 67439-1-PBS

Print datasheet

Synonyms

Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1

验证数据展示

  • WB analysis using 67439-1-Ig (same clone as 67439-1-PBS)

    WB analysis using 67439-1-Ig (same clone as 67439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 67439-1-Ig (same clone as 67439-1-PBS)

    WB analysis of human placenta using 67439-1-Ig (same clone as 67439-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67439-1-Ig (same clone as 67439-1-PBS)

    FC experiment of HeLa using 67439-1-Ig (same clone as 67439-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SOX9 (67439-1-Ig, Clone:1H9A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

产品信息

67439-1-PBS targets SOX9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen SOX9 fusion protein Ag28620 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SRY (sex determining region Y)-box 9
Synonyms Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1
Calculated Molecular Weight 509 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC007951
Gene Symbol SOX9
Gene ID (NCBI) 6662
RRIDAB_2882675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48436
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.