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验证数据展示

  • WB analysis using 67439-1-Ig (same clone as 67439-1-PBS)

    WB analysis using 67439-1-Ig (same clone as 67439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 67439-1-Ig (same clone as 67439-1-PBS)

    WB analysis of human placenta using 67439-1-Ig (same clone as 67439-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67439-1-Ig (SOX9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 67439-1-Ig (same clone as 67439-1-PBS)

    FC experiment of HeLa using 67439-1-Ig (same clone as 67439-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SOX9 (67439-1-Ig, Clone:1H9A6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67439-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SOX9 Monoclonal antibody, PBS Only

SOX9 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 67439-1-PBS

产品说明书

CloneNo. 1H9A6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67439-1-PBS targets SOX9 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 SOX9 fusion protein Ag28620 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SRY (sex determining region Y)-box 9
别名 Transcription factor SOX-9, Transcription factor SOX 9, SRA1, CMPD1, CMD1
计算分子量 509 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC007951
基因名称 SOX9
Gene ID (NCBI) 6662
RRIDAB_2882675
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48436
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SOX9, also named as Transcription factor SOX-9, is a 509 amino acid protein. Sex-determining region Y (SRY)-box 9 protein (SOX9) is a member of the SOX family of transcription factors (TFs) which are developmental regulators that possess high mobility group (HMG) box DNA binding and transactivation domains. It participates in a variety of functions, such as lineage restriction and terminal diferentiation, through precise temporal and spatial expression patterns that difer between particular cell types and tissues. SOX9 gene has been implicated in different types of cancer as an oncogene; however, it also may behave as a tumor suppressor.