Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody
Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241018D12
验证数据展示
经过测试的应用
| Positive WB detected in | PDGF treated HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ribosomal protein S6 |
| Synonyms | P-235/236-rpS6, p RPS6 (Ser235/236), p RPS6, Phospho RPS6 (Ser235/236), phospho rpS6 Ser235/236 |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | NM_001010 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P62753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |