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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human PDGFaa protein

Activity

Typically ≤ 10 ng/mL in 3T3 cell, 150 - 900 ng/mL in NIH 3T3

Species Reactivity

human,mouse,rat

Purity

>95%

Cat No : HZ-1215

Print datasheet

Synonyms

PDGF 1, PDGF A, PDGF subunit A, PDGF1, PDGFA, PDGFaa

Validation Data Gallery

  • Purity of recombinant human PDGFaa was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human PDGFaa was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human PDGFaa (HZ-1215) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFaa for 48hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values are less than 10 ng/mL EC50.

    Recombinant human PDGFaa (HZ-1215) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved with 0.1% FBS for 24 hours before treatment with increasing concentrations of recombinant human PDGFaa for 48hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values are less than 10 ng/mL EC50.

Technical Specifications

GeneID 5154
Species Human
Expression HEK293
Activity Typically ≤ 10 ng/mL in 3T3 cell, 150 - 900 ng/mL in NIH 3T3
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP04085
Molecular Mass 19 and 26 reduced, 35, 40 and 45 kDa non-reduced, homodimer, glycosylated
Formulation 10 mM HOAc, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 10 mM HOAc containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

PDGFaa is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFaa plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for a normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFaa is also involved in oligodendrocyte development and l myelination process in the spinal cord and cerebellum PMID: 29408302; 29282077; 27002148).

Synonyms

PDGF 1, PDGF A, PDGF subunit A, PDGF1, PDGFA, PDGFaa

Publications

SpeciesTitle
rat

Phytomedicine

Kaempferol attenuates carbon tetrachloride (CCl4)-induced hepatic fibrosis by promoting ASIC1a degradation and suppression of the ASIC1a-mediated ERS

Authors - Rui Cao
View Article
human

Oxid Med Cell Longev

Autophagy Contributes to the Maintenance of Genomic Integrity by Reducing Oxidative Stress.

Authors - Wenqing Bu
View Article
mouse

Stem Cell Reports

Conversion of Fibroblast into Functional Leydig-like Cell Using Defined Small Molecules.

Authors - Yan Yang
View Article
mouse

Front Cell Neurosci

Nicotinamide enhances myelin production after demyelination through reduction of astrogliosis and microgliosis

Authors - Stefanos Ioannis Kaplanis
View Article
human

Nat Biotechnol

Morphological diversification and functional maturation of human astrocytes in glia-enriched cortical organoid transplanted in mouse brain

Authors - Meiyan Wang
View Article

Biol Chem

Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy

Authors - Annika Haak
View Article