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Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody, PBS Only

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241018D12

Cat no : 80130-2-PBS

Print datasheet

Synonyms

RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33

验证数据展示

  • WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

    WB analysis using 80130-2-RR (same clone as 80130-2-PBS)

    Non-treated and PDGF treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (RPS6-phospho-S235/236 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80130-2-PBS in a different storage buffer formulation.

产品信息

80130-2-PBS targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S6
Synonyms RPS6, S6, RP S6, ribosomal protein S6, Phosphoprotein NP33
Observed Molecular Weight35 kDa
GenBank Accession NumberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62753
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)