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Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody

Phospho-S6 Ribosomal protein (Ser235/236) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241018D12

Cat no : 80130-2-RR

Print datasheet

Synonyms

P-235/236-rpS6, p RPS6 (Ser235/236), p RPS6, Phospho RPS6 (Ser235/236), phospho rpS6 Ser235/236

验证数据展示

  • IF Staining of HeLa using 80130-2-RR

    IF Staining of HeLa using 80130-2-RR

    Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235/236) antibody (80130-2-RR, Clone: 241018D12) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR004).

  • WB analysis using 80130-2-RR

    WB analysis using 80130-2-RR

    Non-treated and PDGF (HZ-1215) treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236)) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • WB analysis of NIH/3T3 using 80130-2-RR

    WB analysis of NIH/3T3 using 80130-2-RR

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80130-2-RR (Phospho-S6 Ribosomal protein (Ser235/236) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • FC experiment of HeLa using 80130-2-RR

    FC experiment of HeLa using 80130-2-RR

    1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.4 μg Phospho-S6 Ribosomal protein (Ser235/236) Recombinant antibody (80130-2-RR, Clone: 241018D12, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (Cat.NO. RGAR005). Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9, blue) was parallel stained as control. Cells were fixed with 4% PFA .

经过测试的应用

Positive WB detected inPDGF treated HEK-293 cells, PDGF treated C6 cells, PDGF treated NIH/3T3 cells
Positive IF/ICC detected in100 nM Calyculin A (30 minutes) treated HeLa cells
Positive FC (Intra) detected in100 nM Calyculin A (30 minutes) treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy RPS6 Ready-To-Use IHC Kit. RPS6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ribosomal protein S6
Synonyms P-235/236-rpS6, p RPS6 (Ser235/236), p RPS6, Phospho RPS6 (Ser235/236), phospho rpS6 Ser235/236
Observed Molecular Weight35 kDa
GenBank Accession NumberNM_001010
Gene Symbol RPS6
Gene ID (NCBI) 6194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP62753
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)

实验方案

Product Specific Protocols
WB protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RRDownload protocol
IF protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Mol Life Sci

Phosphocholine inhibits proliferation and reduces stemness of endometrial cancer cells by downregulating mTOR-c-Myc signaling

Authors - Kunxiang Gong
View Article