验证数据展示
经过测试的应用
Positive WB detected in | PDGF treated HEK-293 cells, PDGF treated C6 cells, PDGF treated NIH/3T3 cells |
Positive IF/ICC detected in | 100 nM Calyculin A (30 minutes) treated HeLa cells |
Positive FC (Intra) detected in | 100 nM Calyculin A (30 minutes) treated HeLa cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
80130-2-RR targets Phospho-S6 Ribosomal protein (Ser235/236) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | ribosomal protein S6 |
Synonyms | P-235/236-rpS6, p RPS6 (Ser235/236), p RPS6, Phospho RPS6 (Ser235/236), phospho rpS6 Ser235/236 |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | NM_001010 |
Gene Symbol | RPS6 |
Gene ID (NCBI) | 6194 |
RRID | AB_3670467 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P62753 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The ribosomal protein S6 (rpS6) is a component of the small 40S ribosomal subunit implicated in mRNA decoding. rpS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of rpS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). (PMID: 21814187)
实验方案
Product Specific Protocols | |
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WB protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RR | Download protocol |
IF protocol for Phospho-S6 Ribosomal protein (Ser235/236) antibody 80130-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |