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验证数据展示

  • WB analysis using 66251-1-Ig (same clone as 66251-1-PBS)

    WB analysis using 66251-1-Ig (same clone as 66251-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IF Staining of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

    IF Staining of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

14-3-3 Sigma Monoclonal antibody, PBS Only

14-3-3 Sigma Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66251-1-PBS

产品说明书

CloneNo. 3F10D9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SFN Ready-To-Use IHC Kit. SFN primary antibody included.

产品信息

66251-1-PBS targets 14-3-3 Sigma in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 14-3-3 Sigma fusion protein Ag12644 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stratifin
别名 14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC002995
基因名称 14-3-3 Sigma
Gene ID (NCBI) 2810
RRIDAB_2881639
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31947
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.