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14-3-3 Sigma Monoclonal antibody, PBS Only

14-3-3 Sigma Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F10D9

Cat no : 66251-1-PBS

Print datasheet

Synonyms

14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS

验证数据展示

  • WB analysis using 66251-1-Ig (same clone as 66251-1-PBS)

    WB analysis using 66251-1-Ig (same clone as 66251-1-PBS)

    Various cell lysates were subjected to SDS PAGE followed by western blot with 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    IHC staining of human skin using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66251-1-Ig (14-3-3 sigma antibody at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IF Staining of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

    IF Staining of MCF-7 using 66251-1-Ig (same clone as 66251-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 66251-1-Ig (14-3-3 sigma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66251-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SFN Ready-To-Use IHC Kit. SFN primary antibody included.

产品信息

66251-1-PBS targets 14-3-3 Sigma in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen 14-3-3 Sigma fusion protein Ag12644 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name stratifin
Synonyms 14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC002995
Gene Symbol 14-3-3 Sigma
Gene ID (NCBI) 2810
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31947
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.