验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, MCF-7 cells |
| Positive IHC detected in | human skin tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy SFN Ready-To-Use IHC Kit. SFN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66251-1-Ig targets 14-3-3 Sigma in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | 14-3-3 Sigma fusion protein Ag12644 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | stratifin |
| 别名 | 14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS |
| 计算分子量 | 28 kDa |
| 观测分子量 | 28-30 kDa |
| GenBank蛋白编号 | BC002995 |
| 基因名称 | 14-3-3 Sigma |
| Gene ID (NCBI) | 2810 |
| RRID | AB_2881639 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P31947 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| IHC protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| IF protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| FC protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. | ||
Mol Biotechnol Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling | ||
Transl Androl Urol Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples. | ||
Environ Toxicol Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer |
