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验证数据展示

  • IF Staining of MCF-7 using CL555-66251

    IF Staining of MCF-7 using CL555-66251

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®555 14-3-3 Sigma antibody (CL555-66251, Clone: 3F10D9 ) at dilution of 1:200.

  • FC experiment of A431 using CL555-66251

    FC experiment of A431 using CL555-66251

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human 14-3-3 Sigma (CL555-66251, Clone:3F10D9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®555-conjugated 14-3-3 Sigma Monoclonal antibody

14-3-3 Sigma Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66251

产品说明书

CloneNo. 3F10D9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SFN Ready-To-Use IHC Kit. SFN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66251-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66251 targets 14-3-3 Sigma in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 14-3-3 Sigma fusion protein Ag12644 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stratifin
别名 14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC002995
基因名称 14-3-3 Sigma
Gene ID (NCBI) 2810
RRIDAB_2919681
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31947
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

实验方案

Product Specific Protocols
IF protocol for CL555 14-3-3 Sigma antibody CL555-66251Download protocol
FC protocol for CL555 14-3-3 Sigma antibody CL555-66251Download protocol
Standard Protocols
Click here to view our Standard Protocols