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CoraLite®594-conjugated ACE2 Monoclonal antibody

ACE2 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F12A4

Cat No : CL594-66699

Print datasheet

Synonyms

ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15

验证数据展示

  • IF Staining of human kidney using CL594-66699

    IF Staining of human kidney using CL594-66699

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 ACE2 antibody (CL594-66699, Clone: 2F12A4 ) at dilution of 1:200.

  • IF Staining of human kidney using CL594-66699

    IF Staining of human kidney using CL594-66699

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 ACE2 antibody (CL594-66699, Clone: 2F12A4 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman kidney tissue
For other applications, we recommend the unconjugated version of this antibody, 66699-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66699 targets ACE2 in IF applications and shows reactivity with Human samples.

Tested Applications IF-P
Tested Reactivity Human
Immunogen ACE2 fusion protein Ag15554 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Synonyms ACE related carboxypeptidase, ACE2, ACEH, DKFZp434A014, Metalloprotease MPROT15
Calculated Molecular Weight 805 aa, 92 kDa
Observed Molecular Weight
GenBank Accession NumberBC048094
Gene Symbol ACE2
Gene ID (NCBI) 59272
RRIDAB_2920024
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BYF1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2.

实验方案

Product Specific Protocols
IF protocol for CL594 ACE2 antibody CL594-66699Download protocol
Standard Protocols
Click here to view our Standard Protocols