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验证数据展示

  • WB analysis using 67417-1-Ig

    WB analysis using 67417-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67417-1-Ig (ACVR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 67417-1-Ig

    IHC staining of mouse heart using 67417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACVR1 antibody included
  • IHC staining of mouse heart using 67417-1-Ig

    IHC staining of mouse heart using 67417-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67417-1-Ig (ACVR1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ACVR1 antibody included
  • IF Staining of mouse brain using 67417-1-Ig

    IF Staining of mouse brain using 67417-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67417-1-Ig

    IF Staining of mouse brain using 67417-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ACVR1 antibody (67417-1-Ig, Clone: 1F11B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

ACVR1 Monoclonal antibody

ACVR1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67417-1-Ig

产品说明书

CloneNo. 1F11B10
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Pig, Mouse, Rat and More (1)

应用

WB, IHC, IF-P, ELISA

别名

activin A receptor, type I, Activin receptor like kinase 2, Activin receptor type 1, Activin receptor type I, ACTR I, ACTRI, ACVR1, ACVR1A, ACVRLK2, ALK 2, ALK2, FOP, SKR1, TSR I, TSRI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig brain tissue, rat brain tissue, NCI-H1299 cells, mouse brain tissue, JAR cells
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy ACVR1 Ready-To-Use IHC Kit. ACVR1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67417-1-Ig targets ACVR1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Pig, Mouse, Rat
文献引用反应性human
免疫原

CatNo: Ag13508

Product name: Recombinant human ACVR1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 210-509 aa of BC033867

Sequence: LLECVGKGRYGEVWRGSWQGENVAVKIFSSRDEKSWFRETELYNTVMLRHENILGFIASDMTSRHSSTQLWLITHYHEMGSLYDYLQLTTLDTVSCLRIVLSIASGLAHLHIEIFGTQGKPAIAHRDLKSKNILVKKNGQCCIADLGLAVMHSQSTNQLDVGNNPRVGTKRYMAPEVLDETIQVDCFDSYKRVDIWAFGLVLWEVARRMVSNGIVEDYKPPFYDVVPNDPSFEDMRKVVCVDQQRPNIPNRWFSDPTLTSLAKLMKECWYQNPSARLTALRIKKTLTKIDNSLDKLKTDC

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 activin A receptor, type I
别名 activin A receptor, type I, Activin receptor like kinase 2, Activin receptor type 1, Activin receptor type I, ACTR I, ACTRI, ACVR1, ACVR1A, ACVRLK2, ALK 2, ALK2, FOP, SKR1, TSR I, TSRI
计算分子量 509 aa, 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC033867
基因名称 ACVR1
Gene ID (NCBI) 90
RRIDAB_2882657
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ04771
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACVR1 (activin receptor type I), also known as ALK2 or ACTRI, is a receptor for activin. It forms a stable complex with type II receptor after ligand binding. These receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling, and type II receptors are required for binding ligands and for expression of type I receptors. ACVR1 is expressed in many tissues including skeletal muscle and chondrocytes. It functions as a receptor for bone morphogenetic protein (BMP) and induces Indian hedgehog in chondrocytes during skeletal development. Mutations in ACVR1 gene are associated with fibrodysplasia ossificans progressive (PMID: 16642017).

实验方案

Product Specific Protocols
WB protocol for ACVR1 antibody 67417-1-IgDownload protocol
IHC protocol for ACVR1 antibody 67417-1-IgDownload protocol
IF protocol for ACVR1 antibody 67417-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Circulating exosomes repair endothelial cell damage by delivering miR-193a-5p.

Authors - Chang Cao
View Article