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验证数据展示

  • WB analysis of Jurkat using 66677-1-Ig (same clone as 66677-1-PBS)

    WB analysis of Jurkat using 66677-1-Ig (same clone as 66677-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

    WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

    WB result of AKR7A2 antibody (66677-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66677-1-Ig (same clone as 66677-1-PBS)

    IF Staining of HepG2 using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AKR7A2 antibody (66677-1-Ig, Clone: 2E1E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

AKR7A2 Monoclonal antibody, PBS Only

AKR7A2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66677-1-PBS

产品说明书

CloneNo. 2E1E8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66677-1-PBS targets AKR7A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 AKR7A2 fusion protein Ag26105 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
别名 AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase
计算分子量 40 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC007352
基因名称 AKR7A2
Gene ID (NCBI) 8574
RRIDAB_2882031
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43488
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells(PMID:22001351).