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  • Featured Product
  • KD/KO Validated

AKR7A2 Monoclonal antibody, PBS Only

AKR7A2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E1E8

Cat no : 66677-1-PBS

Print datasheet

Synonyms

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

验证数据展示

  • WB analysis of Jurkat using 66677-1-Ig (same clone as 66677-1-PBS)

    WB analysis of Jurkat using 66677-1-Ig (same clone as 66677-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

    WB analysis of HEK-293 using 66677-1-Ig (same clone as 66677-1-PBS)

    WB result of AKR7A2 antibody (66677-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    IHC staining of human liver cancer using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66677-1-Ig (same clone as 66677-1-PBS)

    IF Staining of HepG2 using 66677-1-Ig (same clone as 66677-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AKR7A2 antibody (66677-1-Ig, Clone: 2E1E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66677-1-PBS in a different storage buffer formulation.

产品信息

66677-1-PBS targets AKR7A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen AKR7A2 fusion protein Ag26105 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Synonyms AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC007352
Gene Symbol AKR7A2
Gene ID (NCBI) 8574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43488
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells(PMID:22001351).