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AKR7A2 Monoclonal antibody

AKR7A2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E1E8

Cat no : 66677-1-Ig

Print datasheet

Synonyms

AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase

验证数据展示

  • WB analysis of Jurkat using 66677-1-Ig

    WB analysis of Jurkat using 66677-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66677-1-Ig (AKR7A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66677-1-Ig

    WB analysis of HEK-293 using 66677-1-Ig

    WB result of AKR7A2 antibody (66677-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKR7A2 transfected HEK-293 cells.

  • IHC staining of human liver cancer using 66677-1-Ig

    IHC staining of human liver cancer using 66677-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66677-1-Ig

    IHC staining of human liver cancer using 66677-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66677-1-Ig (AKR7A2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 66677-1-Ig

    IF Staining of HepG2 using 66677-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AKR7A2 antibody (66677-1-Ig, Clone: 2E1E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66677-1-Ig targets AKR7A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen AKR7A2 fusion protein Ag26105 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase)
Synonyms AFAR, AFAR1, AFB1 aldehyde reductase 1, AFB1 AR 1, AFB1 AR1, AKR7, AKR7A2, Aldoketoreductase 7, SSA reductase
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC007352
Gene Symbol AKR7A2
Gene ID (NCBI) 8574
RRIDAB_2882031
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43488
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells(PMID:22001351).

实验方案

Product Specific Protocols
WB protocol for AKR7A2 antibody 66677-1-IgDownload protocol
IHC protocol for AKR7A2 antibody 66677-1-IgDownload protocol
IF protocol for AKR7A2 antibody 66677-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Chem Biol Interact

Aldo-keto reductase-7A2 protects against atorvastatin-induced hepatotoxicity via Nrf2 activation

Authors - Dan Li
View Article