验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 66677-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66677 targets AKR7A2 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | AKR7A2 fusion protein Ag26105 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) |
| 别名 | AKR7, AFB1-AR 1, AFB1 AR1, AFB1 AR 1, AFAR |
| 计算分子量 | 40 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC007352 |
| 基因名称 | AKR7A2 |
| Gene ID (NCBI) | 8574 |
| RRID | AB_2919355 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O43488 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKR7A2(Aflatoxin B1 aldehyde reductase member 2) is also named as AFAR, AFAR1, AKR7 and belongs to the aldo/keto reductase family. AKR7A2 enzyme is catalytically active toward aldehydes arising from lipid peroxidation, suggesting a potential role against the consequences of oxidative stress, and representing an important detoxication route in mammalian cells(PMID:22001351).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AKR7A2 antibody CL488-66677 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |