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验证数据展示

  • WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

    WB analysis using 67453-1-Ig (same clone as 67453-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67453-1-Ig (ALDOA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

    IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDOA antibody included
  • IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

    IHC staining of human lung cancer using 67453-1-Ig (same clone as 67453-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67453-1-Ig (ALDOA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ALDOA antibody included
  • IF Staining of HeLa using 67453-1-Ig (same clone as 67453-1-PBS)

    IF Staining of HeLa using 67453-1-Ig (same clone as 67453-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDOA antibody (67453-1-Ig, Clone: 1D6C2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67453-1-PBS in a different storage buffer formulation.

ALDOA Monoclonal antibody, PBS Only

ALDOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67453-1-PBS

产品说明书

CloneNo. 1D6C2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, ELISA

别名

Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ALDOA Ready-To-Use IHC Kit. ALDOA primary antibody included.

产品信息

67453-1-PBS targets ALDOA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag17952

Product name: Recombinant human ALDOA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2-364 aa of BC016800

Sequence: PYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 aldolase A, fructose-bisphosphate
别名 Lung cancer antigen NY-LU-1, Fructose-bisphosphate aldolase A, EC:4.1.2.13, Aldolase A, ALDA
计算分子量 39 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC016800
基因名称 ALDOA
Gene ID (NCBI) 226
RRIDAB_2882687
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04075
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALDOA(Fructose-bisphosphate aldolase A) is also named as lung cancer antigen NY-LU-1,muscle-type aldolase,ALDA and belongs to the class I fructose-bisphosphate aldolase family.The ALDOA gene encodes a homotetrameric protein(PMID:14615364).It is found in skeletal muscle and red blood cells, and functions in glycolysis, promoting the reversible cleavage of FBP to triose phosphate, G-3-P and DHAP. ALDOA is upregulated in various cancers, including human lung squamous and renal cell carcinomas(PMID:19937139).