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  • Featured Product
  • KD/KO Validated

APP Monoclonal antibody, PBS Only

APP Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5C2A1

Cat no : 60342-1-PBS

Print datasheet

Synonyms

amyloid precursor protein, Alzheimer disease amyloid A4 protein homolog, AD1, AB42, AB40

验证数据展示

  • WB analysis of Y79 using 60342-1-Ig (same clone as 60342-1-PBS)

    WB analysis of Y79 using 60342-1-Ig (same clone as 60342-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 60342-1-Ig (APP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

    IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

    IHC staining of human gliomas using 60342-1-Ig (same clone as 60342-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60342-1-Ig (APP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60342-1-PBS in a different storage buffer formulation.

产品信息

60342-1-PBS targets APP in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat, pig, rabbit
Immunogen APP fusion protein Ag0769 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name amyloid beta (A4) precursor protein
Synonyms amyloid precursor protein, Alzheimer disease amyloid A4 protein homolog, AD1, AB42, AB40
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC004369
Gene Symbol APP
Gene ID (NCBI) 351
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05067
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).