验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, HEK-293 cells, Y79 cells, rabbit brain tissue, rat brain tissue |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60342-1-Ig targets APP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP |
| Tested Reactivity | human, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat |
| Immunogen | APP fusion protein Ag0769 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | amyloid beta (A4) precursor protein |
| Synonyms | amyloid precursor protein, Alzheimer disease amyloid A4 protein homolog, AD1, AB42, AB40 |
| Calculated Molecular Weight | 87 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC004369 |
| Gene Symbol | APP |
| Gene ID (NCBI) | 351 |
| RRID | AB_2881451 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05067 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Aβ derives from APP via proteolytic cleavage by proteases called α-, β- and γ-secretase. The α-secretase cleavage precludes the formation of Aβ, while the β- and γ-cleavages generate APP components with amyloidogenic features. Amyloid beta A4 precursor protein(APP), encoded by APP gene which locate on human chromosome 21q, is a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. APP expressed in all fetal tissues and is pronounced in brain, kidney, heart and spleen, but weak in liver. Defects in APP are the cause of Alzheimer disease type 1 (AD1).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for APP antibody 60342-1-Ig | Download protocol |
| IHC protocol for APP antibody 60342-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Bone Marrow-Derived GCA+ Immune Cells Drive Alzheimer's Disease Progression | ||
Aging Cell Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model. | ||
Protein Cell Metformin activates chaperone-mediated autophagy and improves disease pathologies in an Alzheimer disease mouse model. | ||
Cell Rep CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease. | ||
Aging (Albany NY) Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease. | ||
Front Aging Neurosci Agomelatine Prevents Amyloid Plaque Deposition, Tau Phosphorylation, and Neuroinflammation in APP/PS1 Mice.
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