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  • Featured Product
  • KD/KO Validated

AZI1 Polyclonal antibody

AZI1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 25735-1-AP

Print datasheet

Synonyms

5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118

验证数据展示

  • WB analysis of mouse testis using 25735-1-AP

    WB analysis of mouse testis using 25735-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25735-1-AP (AZI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25735-1-AP

    IF Staining of HeLa using 25735-1-AP

    Immunofluorescent analysis of HeLa cells using 25735-1-AP (AZI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25735-1-AP is AZI1 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen AZI1 fusion protein Ag22699 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5-azacytidine induced 1
Synonyms 5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118
Calculated Molecular Weight 1083 aa, 122 kDa
Observed Molecular Weight 131 kDa
GenBank Accession NumberBC136578
Gene Symbol AZI1
Gene ID (NCBI) 22994
RRIDAB_2880216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPN4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

实验方案

Product Specific Protocols
WB protocol for AZI1 antibody 25735-1-APDownload protocol
IF protocol for AZI1 antibody 25735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
View Article
humanWB,IF,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
  • KD Validated
View Article
human,mouseIF

Elife

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

Authors - Emma A Hall
View Article
humanIF

PLoS Biol

Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.

Authors - Özge Z Aydin
View Article
humanWB

Front Oncol

USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.

Authors - Catherine M Nickson
  • KD Validated
View Article
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
View Article