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验证数据展示

  • WB analysis of mouse testis using 25735-1-AP

    WB analysis of mouse testis using 25735-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25735-1-AP (AZI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 25735-1-AP

    IF Staining of HeLa using 25735-1-AP

    Immunofluorescent analysis of HeLa cells using 25735-1-AP (AZI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

AZI1 Polyclonal antibody

AZI1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25735-1-AP

产品说明书

引用文献(13)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA and More (2)

别名

5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25735-1-AP targets AZI1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 AZI1 fusion protein Ag22699 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5-azacytidine induced 1
别名 5 azacytidine induced 1, AZ1, AZI1, Centrosomal protein of 131 kDa, Cep131, KIAA1118
计算分子量 1083 aa, 122 kDa
观测分子量 131 kDa
GenBank蛋白编号BC136578
基因名称 AZI1
Gene ID (NCBI) 22994
RRIDAB_2880216
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPN4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

实验方案

Product Specific Protocols
WB protocol for AZI1 antibody 25735-1-APDownload protocol
IF protocol for AZI1 antibody 25735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

BioE3 identifies specific substrates of ubiquitin E3 ligases

Authors - Orhi Barroso-Gomila
View Article
humanWB,IF,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
  • KD Validated
View Article
human,mouseIF

Elife

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

Authors - Emma A Hall
View Article
humanIF

PLoS Biol

Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.

Authors - Özge Z Aydin
View Article
humanWB

Front Oncol

USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.

Authors - Catherine M Nickson
  • KD Validated
View Article
humanIF

RNA

Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.

Authors - Alan L Jiao
View Article