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验证数据展示

  • FC experiment of HeLa using CL488-25735

    FC experiment of HeLa using CL488-25735

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AZI1 (CL488-25735) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated AZI1 Polyclonal antibody

AZI1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-25735

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

Pre-acrosome localization protein 1, KIAA1118, CEP131, AZ1, 5-azacytidine-induced protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 25735-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25735 targets AZI1 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 AZI1 fusion protein Ag22699 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 5-azacytidine induced 1
别名 Pre-acrosome localization protein 1, KIAA1118, CEP131, AZ1, 5-azacytidine-induced protein 1
计算分子量 1083 aa, 122 kDa
观测分子量 131 kDa
GenBank蛋白编号BC136578
基因名称 AZI1
Gene ID (NCBI) 22994
RRIDAB_2919204
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPN4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 AZI1 antibody CL488-25735Download protocol
Standard Protocols
Click here to view our Standard Protocols