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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AZI1 Polyclonal antibody

AZI1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-25735

Print datasheet

Synonyms

Pre-acrosome localization protein 1, KIAA1118, CEP131, AZ1, 5-azacytidine-induced protein 1

验证数据展示

  • FC experiment of HeLa using CL488-25735

    FC experiment of HeLa using CL488-25735

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human AZI1 (CL488-25735) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 25735-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25735 targets AZI1 in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen AZI1 fusion protein Ag22699 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name 5-azacytidine induced 1
Synonyms Pre-acrosome localization protein 1, KIAA1118, CEP131, AZ1, 5-azacytidine-induced protein 1
Calculated Molecular Weight 1083 aa, 122 kDa
Observed Molecular Weight 131 kDa
GenBank Accession NumberBC136578
Gene Symbol AZI1
Gene ID (NCBI) 22994
RRIDAB_2919204
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPN4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 AZI1 antibody CL488-25735Download protocol
Standard Protocols
Click here to view our Standard Protocols