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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated BCL10 Polyclonal antibody

BCL10 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-50180

Print datasheet

Synonyms

CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10

验证数据展示

  • IF Staining of A549 using CL647-50180

    IF Staining of A549 using CL647-50180

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 647 BCL10 antibody (CL647-50180) at dilution of 1:200.

  • FC experiment of Jurkat using CL647-50180

    FC experiment of Jurkat using CL647-50180

    1X10^6 Jurkat cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human BCL10 (CL647-50180) (red), or 0.8 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL10 Ready-To-Use IHC Kit. BCL10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 50180-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-50180 targets BCL10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen BCL10 fusion protein Ag0037 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell CLL/lymphoma 10
Synonyms CARD-containing molecule enhancing NF-kappa-B, c E10, Bcl-10, Bcl 10, B-cell lymphoma/leukemia 10
Calculated Molecular Weight 233 aa, 26 kDa
Observed Molecular Weight 26-32 kDa
GenBank Accession NumberBC053617
Gene Symbol BCL10
Gene ID (NCBI) 8915
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95999
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The gene encoding BCL10 was identified by its recurrent translocation t(1;14)(p22;q32) in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. BCL10 contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 BCL10 antibody CL647-50180Download protocol
Standard Protocols
Click here to view our Standard Protocols