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C9orf72 Monoclonal antibody, PBS Only

C9orf72 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D2H6

Cat no : 66140-1-PBS

Print datasheet

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 3D2H6

验证数据展示

  • IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    IHC staining of human gliomas using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
  • WB analysis of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    WB analysis of human brain using 66140-1-Ig (same clone as 66140-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • IP experiment of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

    IP experiment of C6 using 66140-1-Ig (same clone as 66140-1-PBS)

    IP result of anti-C9orf72 (IP:66140-1-Ig, 4ug; Detection:66140-1-Ig 1:1000) with C6 cells lysate 1320ug. This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 66140-1-Ig (same clone as 66140-1-PBS)

    IF Staining of SH-SY5Y using 66140-1-Ig (same clone as 66140-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using C9orf72 antibody (66140-1-Ig, Clone: 3D2H6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66140-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.

产品信息

66140-1-PBS targets C9orf72 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen C9orf72 fusion protein Ag21080 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chromosome 9 open reading frame 72
Synonyms Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 3D2H6
Calculated Molecular Weight 481 aa, 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC020851
Gene Symbol C9orf72
Gene ID (NCBI) 203228
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96LT7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.