C9orf72 Monoclonal antibody
C9orf72 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3D2H6
验证数据展示
经过测试的应用
Positive WB detected in | human brain tissue, C6 cells, Neuro-2a cells |
Positive IP detected in | C6 cells |
Positive IHC detected in | human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 66140-1-Ig is C9orf72 Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | C9orf72 fusion protein Ag21080 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | chromosome 9 open reading frame 72 |
Synonyms | C9RANT |
Calculated Molecular Weight | 481 aa, 54 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC020851 |
Gene Symbol | C9orf72 |
Gene ID (NCBI) | 203228 |
RRID | AB_2784547 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96LT7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
IHC protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
IF protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
IP protocol for C9orf72 antibody 66140-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Neuropathol Commun Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers. | ||
Sci Rep Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo. | ||
Amyotroph Lateral Scler Frontotemporal Degener Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies. | ||
Acta Neuropathol Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis. | ||
Animal Model Exp Med Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats. | ||