CoraLite®594-conjugated C9orf72 Monoclonal antibody
C9orf72 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3D2H6
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SH-SY5Y cells |
Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66140-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66140 targets C9orf72 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | C9orf72 fusion protein Ag21080 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | chromosome 9 open reading frame 72 |
| Synonyms | C9orf72, RP11 27J8.2 |
| Calculated Molecular Weight | 481 aa, 54 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC020851 |
| Gene Symbol | C9orf72 |
| Gene ID (NCBI) | 203228 |
| RRID | AB_2883523 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96LT7 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 C9orf72 antibody CL594-66140 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |