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CoraLite® Plus 488-conjugated C9orf72 Monoclonal antibody

C9orf72 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3D2H6

Cat no : CL488-66140

Print datasheet

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 3D2H6

验证数据展示

  • IF Staining of mouse brain using CL488-66140

    IF Staining of mouse brain using CL488-66140

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488-conjugated C9orf72 antibody (CL488-66140, Clone: 3D2H6 ) at dilution of 1:100.

  • IF Staining of SH-SY5Y using CL488-66140

    IF Staining of SH-SY5Y using CL488-66140

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CL488-66140 (C9orf72 antibody) at dilution of 1:50.

经过测试的应用

Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66140-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66140 targets C9orf72 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen C9orf72 fusion protein Ag21080 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chromosome 9 open reading frame 72
Synonyms Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 3D2H6
Calculated Molecular Weight 481 aa, 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC020851
Gene Symbol C9orf72
Gene ID (NCBI) 203228
RRIDAB_2883271
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96LT7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 C9orf72 antibody CL488-66140Download protocol
Standard Protocols
Click here to view our Standard Protocols