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C9orf72 Monoclonal antibody

C9orf72 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F9A2

Cat no : 67824-1-Ig

Print datasheet

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2

验证数据展示

  • WB analysis using 67824-1-Ig

    WB analysis using 67824-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • FC experiment of HEK-293T using 67824-1-Ig

    FC experiment of HEK-293T using 67824-1-Ig

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human C9orf72 (67824-1-Ig, Clone:2F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis of Neuro-2a using 67824-1-Ig

    WB analysis of Neuro-2a using 67824-1-Ig

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 67824-1-Ig

    IHC staining of mouse lung using 67824-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of mouse intestine using 67824-1-Ig

    IHC staining of mouse intestine using 67824-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of rat kidney using 67824-1-Ig

    IHC staining of rat kidney using 67824-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C9orf72 antibody included

经过测试的应用

Positive WB detected inpig brain tissue, HEK-293 cells, U-87 MG cells, Neuro-2a cells, PC-12 cells, C6 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse lung tissue, mouse intestine, rat kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293T cells
Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67824-1-Ig targets C9orf72 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen C9orf72 fusion protein Ag21080 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chromosome 9 open reading frame 72
Synonyms Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2
Calculated Molecular Weight 481 aa, 54 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC020851
Gene Symbol C9orf72
Gene ID (NCBI) 203228
RRIDAB_2918586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96LT7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.

实验方案

Product Specific Protocols
WB protocol for C9orf72 antibody 67824-1-IgDownload protocol
IHC protocol for C9orf72 antibody 67824-1-IgDownload protocol
FC protocol for C9orf72 antibody 67824-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols