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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67824-1-Ig (same clone as 67824-1-PBS)

    WB analysis using 67824-1-Ig (same clone as 67824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 67824-1-Ig (same clone as 67824-1-PBS)

    FC experiment of HEK-293T using 67824-1-Ig (same clone as 67824-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human C9orf72 (67824-1-Ig, Clone:2F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

  • WB analysis of Neuro-2a using 67824-1-Ig (same clone as 67824-1-PBS)

    WB analysis of Neuro-2a using 67824-1-Ig (same clone as 67824-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67824-1-Ig (same clone as 67824-1-PBS)

    IHC staining of mouse lung using 67824-1-Ig (same clone as 67824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of mouse intestine using 67824-1-Ig (same clone as 67824-1-PBS)

    IHC staining of mouse intestine using 67824-1-Ig (same clone as 67824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of rat kidney using 67824-1-Ig (same clone as 67824-1-PBS)

    IHC staining of rat kidney using 67824-1-Ig (same clone as 67824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included

C9orf72 Monoclonal antibody, PBS Only

C9orf72 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 67824-1-PBS

产品说明书

CloneNo. 2F9A2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.

产品信息

67824-1-PBS targets C9orf72 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 C9orf72 fusion protein Ag21080 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 chromosome 9 open reading frame 72
别名 Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2
计算分子量 481 aa, 54 kDa
观测分子量50 kDa
GenBank蛋白编号BC020851
基因名称 C9orf72
Gene ID (NCBI) 203228
RRIDAB_2918586
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96LT7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.