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C9orf72 Monoclonal antibody, PBS Only

C9orf72 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F9A2

Cat no : 67824-1-PBS

Print datasheet

Synonyms

Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2

验证数据展示

  • WB analysis using 67824-1-Ig (same clone as 67824-1-PBS)

    WB analysis using 67824-1-Ig (same clone as 67824-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293T using 67824-1-Ig (same clone as 67824-1-PBS)

    FC experiment of HEK-293T using 67824-1-Ig (same clone as 67824-1-PBS)

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human C9orf72 (67824-1-Ig, Clone:2F9A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

  • WB analysis of Neuro-2a using 67824-1-Ig (same clone as 67824-1-PBS)

    WB analysis of Neuro-2a using 67824-1-Ig (same clone as 67824-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67824-1-Ig (C9orf72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 67824-1-Ig (same clone as 67824-1-PBS)

    IHC staining of mouse lung using 67824-1-Ig (same clone as 67824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of mouse intestine using 67824-1-Ig (same clone as 67824-1-PBS)

    IHC staining of mouse intestine using 67824-1-Ig (same clone as 67824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
  • IHC staining of rat kidney using 67824-1-Ig (same clone as 67824-1-PBS)

    IHC staining of rat kidney using 67824-1-Ig (same clone as 67824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67824-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67824-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with C9orf72 antibody included
Planning an IHC experiment? We recommend our IHCeasy C9orf72 Ready-To-Use IHC Kit. C9orf72 primary antibody included.

产品信息

67824-1-PBS targets C9orf72 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen C9orf72 fusion protein Ag21080 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name chromosome 9 open reading frame 72
Synonyms Guanine nucleotide exchange factor C9orf72, DENNL72, DENND9, C9RANT, 2F9A2
Calculated Molecular Weight 481 aa, 54 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC020851
Gene Symbol C9orf72
Gene ID (NCBI) 203228
RRIDAB_2918586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96LT7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.