验证数据展示
经过测试的应用
| Positive WB detected in | HT-1080 cells, HeLa cells, HepG2 cells, Jurkat cells, THP-1 cells, MDA-MB-231 cells, JAR cells, K-562 cells, U-937 cells |
| Positive IHC detected in | human liver cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue, human liver tissue |
This antibody is not recommended for immunocytofluorescent assays.
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®532 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67300-1-Ig targets CD107a / LAMP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CD107a / LAMP1 fusion protein Ag28869 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lysosomal-associated membrane protein 1 |
| 别名 | CD107a, LAMP1, LAMP-1, LAMP 1, CD107 antigen-like family member A |
| 计算分子量 | 45 kDa |
| 观测分子量 | 90-120 kDa |
| GenBank蛋白编号 | BC021288 |
| 基因名称 | LAMP1 |
| Gene ID (NCBI) | 3916 |
| ENSEMBL Gene ID | ENSG00000185896 |
| RRID | AB_2882564 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P11279 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
| IHC protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
| IF protocol for CD107a / LAMP1 antibody 67300-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Generation and Characterization of SORT1-Targeted Antibody-Drug Conjugate for the Treatment of SORT1-Positive Breast Tumor | ||
Int J Mol Sci Polymeric Polylactic Acid-Glycolic Acid-Based Nanoparticles Deliver Nintedanib Across the Blood-Brain Barrier to Inhibit Glioblastoma Growth | ||
Cancers (Basel) A Novel Phenazine Analog, CPUL1, Suppresses Autophagic Flux and Proliferation in Hepatocellular Carcinoma: Insight from Integrated Transcriptomic and Metabolomic Analysis | ||
MedComm (2020) Leucine-rich repeat kinase 2 (LRRK2) inhibition upregulates microtubule-associated protein 1B to ameliorate lysosomal dysfunction and parkinsonism | ||
J Nanobiotechnology A novel "prime and pull" strategy mediated by the combination of two dendritic cell-targeting designs induced protective lung tissue-resident memory T cells against H1N1 influenza virus challenge | ||
Antioxidants (Basel) Stimulation of Hepatic Ferritinophagy Mitigates Irp2 Depletion-Induced Anemia |
