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CD107a / LAMP1 Monoclonal antibody, PBS Only

CD107a / LAMP1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B2E9

Cat no : 67300-1-PBS

Print datasheet

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, CD107 antigen-like family member A

验证数据展示

  • WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    WB analysis using 67300-1-Ig (same clone as 67300-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67300-1-Ig (CD107a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IHC staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67300-1-Ig (CD107a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LAMP1/CD107a antibody included
  • IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    IF Staining of human liver cancer using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD107a antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 67300-1-Ig (same clone as 67300-1-PBS)

    IF Staining of human liver using 67300-1-Ig (same clone as 67300-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human liver tissue using CD107a / LAMP1 antibody (67300-1-Ig, Clone: 3B2E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67300-1-PBS in a different storage buffer formulation.

This antibody is not recommended for immunocytofluorescent assays.

Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.

产品信息

67300-1-PBS targets CD107a / LAMP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD107a / LAMP1 fusion protein Ag28869 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lysosomal-associated membrane protein 1
Synonyms CD107a, LAMP1, LAMP-1, LAMP 1, CD107 antigen-like family member A
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 90-120 kDa
GenBank Accession NumberBC021288
Gene Symbol LAMP1
Gene ID (NCBI) 3916
ENSEMBL Gene IDENSG00000185896
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11279
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.