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验证数据展示

  • FC experiment of NIH/3T3 using CL568-65050

    FC experiment of NIH/3T3 using CL568-65050

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®568 Anti-Mouse CD107a / LAMP1 (CL568-65050, Clone:1D4B) (red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of NIH/3T3 using CL568-65050

    IF Staining of NIH/3T3 using CL568-65050

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite®568 CD107a antibody (CL568-65050, Clone: 1D4B ) at dilution of 1:100.

CoraLite®568 Anti-Mouse CD107a / LAMP1 (1D4B)

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL568-65050

产品说明书

CloneNo. 1D4B

宿主/亚型

Rat / IgG2a, kappa

种属反应性

Mouse

应用

IF/ICC, FC (Intra)

别名

AI196048, CD107a, Lamp 1, Lamp1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®568
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Positive FC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 65050-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL568-65050 targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with Mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Mouse
免疫原 Plasma membrane fraction of mouse embryo NIH/3T3 cell line 种属同源性预测
宿主/亚型 Rat / IgG2a, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 lysosomal-associated membrane protein 1
别名 AI196048, CD107a, Lamp 1, Lamp1
GenBank蛋白编号BC006785
基因名称 CD107a
Gene ID (NCBI) 16783
RRIDAB_2919714
偶联类型 CoraLite®568 Fluorescent Dye
最大激发/发射波长568 nm / 587 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP11438
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

实验方案

Product Specific Protocols
IF protocol for CL568 CD107a / LAMP1 antibody CL568-65050Download protocol
Standard Protocols
Click here to view our Standard Protocols