CoraLite® Plus 647 Anti-Human CD4 (OKT4)
CD4 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG2b, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
OKT4
验证数据展示
经过测试的应用
Positive FC detected in | human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 65134-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65134 targets CD4 in FC applications and shows reactivity with Human samples.
Tested Applications | FC Application Description |
Tested Reactivity | Human |
Immunogen | Human peripheral T cells 种属同源性预测 |
Host / Isotype | Mouse / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | CD4 molecule |
Synonyms | CD4, CD4 molecule, CD4mut |
Calculated Molecular Weight | 55 kDa |
GenBank Accession Number | BC025782 |
Gene Symbol | CD4 |
Gene ID (NCBI) | 920 |
ENSEMBL Gene ID | ENSG00000010610 |
RRID | AB_2883701 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P01730 |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 647 CD4 antibody CL647-65134 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |