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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65134-1-Ig

    FC experiment of human PBMCs using 65134-1-Ig

    1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65134-1-Ig, Clone:OKT4) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65134-1-Ig

    FC experiment of human PBMCs using 65134-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65134-1-Ig, Clone:OKT4) and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Lymphocytes were gated.

Anti-Human CD4 (OKT4)

CD4 Monoclonal Antibody for FC
Cat No. 65134-1-Ig

Print datasheet

CloneNo. OKT4

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

Human

Applications

FC

Synonyms

CD4, CD4 molecule, CD4mut

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65134-1-Ig targets CD4 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human peripheral T cells 种属同源性预测
Host / Isotype Mouse / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD4 molecule
Synonyms CD4, CD4 molecule, CD4mut
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_2881474
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01730
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CD4 antibody 65134-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols