CoraLite® Plus 488 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody
CD44 Uni-rAbTM Recombinant Antibody for FC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
F10-44-2
验证数据展示
经过测试的应用
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65608-1-MR
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65608 targets CD44 in FC applications and shows reactivity with human samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | human |
| Immunogen | N/A 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Full Name | CD44 molecule (Indian blood group) |
| Synonyms | Epican, ECMR-III, ECMR III, CSPG8, CDW44 |
| Calculated Molecular Weight | 742 aa, 82 kDa |
| GenBank Accession Number | BC004372 |
| Gene Symbol | CD44 |
| Gene ID (NCBI) | 960 |
| ENSEMBL Gene ID | ENSG00000026508 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD44 antibody CL488-65608 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |