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Species Reactivity
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验证数据展示

  • FC experiment of human PBMCs using CL488-65608

    FC experiment of human PBMCs using CL488-65608

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody (CL488-65608, Clone: F10-44-2), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL488-65608

    FC experiment of human PBMCs using CL488-65608

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody (CL488-65608, Clone: F10-44-2) (red), or CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

CoraLite® Plus 488 Anti-Human CD44 (F10-44-2) Mouse IgG2a Recombinant Antibody

CD44 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL488-65608
CloneNo. F10-44-2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Epican, ECMR-III, ECMR III, CSPG8, CDW44

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65608-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65608 targets CD44 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD44 molecule (Indian blood group)
Synonyms Epican, ECMR-III, ECMR III, CSPG8, CDW44
Calculated Molecular Weight 742 aa, 82 kDa
GenBank Accession NumberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
RRIDAB_3672900
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CD44 antibody CL488-65608Download protocol
Standard Protocols
Click here to view our Standard Protocols