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CRP Monoclonal antibody, PBS Only

CRP Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F10A11

Cat No : 66250-1-PBS

Print datasheet

Synonyms

C reactive protein, C-Reactive Protein, CRP, PTX1

验证数据展示

  • WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (CRP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRP antibody included
  • IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (CRP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRP antibody included
  • IF Staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    IF Staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CRP Ready-To-Use IHC Kit. CRP primary antibody included.

产品信息

66250-1-PBS targets CRP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen CRP fusion protein Ag9883 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name C-reactive protein, pentraxin-related
Synonyms C reactive protein, C-Reactive Protein, CRP, PTX1
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC020766
Gene Symbol CRP
Gene ID (NCBI) 1401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02741
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.