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  • Featured Product
  • KD/KO Validated

C-Reactive Protein/CRP Monoclonal antibody, PBS Only

C-Reactive Protein/CRP Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F10A11

Cat no : 66250-1-PBS

Print datasheet

Synonyms

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

验证数据展示

  • WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:2000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    WB analysis of serum from mouse injected with bacteria using 66250-1-Ig (same clone as 66250-1-PBS)

    Serum from normal mouse (left) and mouse injected with bacteria (right) were subjected to SDS PAGE followed by western blot with 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:1000. Horseradish peroxidase conjugated protein A was used for signal developing instead of secondary antibody incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRP antibody included
  • IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    IHC staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66250-1-Ig (C-Reactive Protein/CRP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CRP antibody included
  • IF Staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    IF Staining of human liver using 66250-1-Ig (same clone as 66250-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using 66250-1-Ig (CRP antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66250-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CRP Ready-To-Use IHC Kit. CRP primary antibody included.

产品信息

66250-1-PBS targets C-Reactive Protein/CRP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen C-Reactive Protein/CRP fusion protein Ag9883 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name C-reactive protein, pentraxin-related
Synonyms CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC020766
Gene Symbol CRP
Gene ID (NCBI) 1401
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02741
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.