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C-Reactive Protein/CRP Monoclonal antibody

C-Reactive Protein/CRP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66250-1-Ig

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CloneNo. 3F10A11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF-P, ELISA and More (1)

CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inserum from mouse injected with bacteria, pig liver tissue, rat liver tissue, serum from mouse injected with bacteria tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy CRP Ready-To-Use IHC Kit. CRP primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66250-1-Ig targets C-Reactive Protein/CRP in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat, monkey
Immunogen C-Reactive Protein/CRP fusion protein Ag9883 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name C-reactive protein, pentraxin-related
Synonyms CRP, PTX1, C-reactive protein(1-205), C-Reactive Protein, C reactive protein
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC020766
Gene Symbol CRP
Gene ID (NCBI) 1401
RRIDAB_2881638
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02741
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

实验方案

Product Specific Protocols
WB protocol for C-Reactive Protein/CRP antibody 66250-1-IgDownload protocol
IHC protocol for C-Reactive Protein/CRP antibody 66250-1-IgDownload protocol
IF protocol for C-Reactive Protein/CRP antibody 66250-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Nat Commun

Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model

Authors - Mengyan Hu
humanIF

Front Immunol

Expression and clinical significance of interleukin-6 pathway in cholangiocarcinoma

Authors - Dongqing Gu
humanIHC

J Cell Mol Med

Proteomic studies in VWA1-related neuromyopathy allowed new pathophysiological insights and the definition of blood biomarkers

Authors - Mohammed Athamneh
mouseWB

Front Immunol

Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.

Authors - Anika Alberts

Sci Rep

A label-free fiber optic SPR biosensor for specific detection of C-reactive protein.

Authors - Wenjia Wang
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
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