CRP Monoclonal antibody

CRP Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3F10A11

Cat No : 66250-1-Ig

Print datasheet

Synonyms

C reactive protein, CRP, PTX1



经过测试的应用

Positive WB detected inserum from mouse injected with bacteria, pig liver tissue, rat liver tissue, serum from mouse injected with bacteria tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

66250-1-Ig targets CRP in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF, IHC, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CRP fusion protein Ag9883
Full Name C-reactive protein, pentraxin-related
Synonyms C reactive protein, CRP, PTX1
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC020766
Gene symbol CRP
UniprotP02741
Gene ID (NCBI) 1401
RRIDAB_2881638
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

实验方案

Product Specific Protocols
WB protocol for CRP antibody 66250-1-IgDownload protocol
IHC protocol for CRP antibody 66250-1-IgDownload protocol
IF protocol for CRP antibody 66250-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IHC

Brain Behav

Prenatal stress induced depressive-like behavior and region dependently high CRP level in offspring rats.

Authors - Shaoning Li
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts

Sci Rep

A label-free fiber optic SPR biosensor for specific detection of C-reactive protein.

Authors - Wenjia Wang
mouseWB

Front Immunol

Binding of Macrophage Receptor MARCO, LDL, and LDLR to Disease-Associated Crystalline Structures.

Authors - Anika Alberts