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CoraLite® Plus 488-conjugated CRP Monoclonal antibody

CRP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F10A11

Cat No : CL488-66250

Print datasheet

Synonyms

C reactive protein, CRP, PTX1

验证数据展示

  • IF Staining of human liver using CL488-66250

    IF Staining of human liver using CL488-66250

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL488-66250 (CRP antibody) at dilution of 1:100.

  • IF Staining of human liver using CL488-66250

    IF Staining of human liver using CL488-66250

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using CL488-66250 (CRP antibody) at dilution of 1:100.

经过测试的应用

Positive IF detected inhuman liver tissue
Planning an IHC experiment? We recommend our IHCeasy CRP Ready-To-Use IHC Kit. CRP primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66250-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66250 targets CRP in IF applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat, pig
Immunogen CRP fusion protein Ag9883 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name C-reactive protein, pentraxin-related
Synonyms C reactive protein, CRP, PTX1
Calculated Molecular Weight 224 aa, 25 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC020766
Gene Symbol CRP
Gene ID (NCBI) 1401
RRIDAB_2883294
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02741
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CRP antibody CL488-66250Download protocol
Standard Protocols
Click here to view our Standard Protocols