• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Caspase 9/p35/p10 Monoclonal antibody, PBS Only

Caspase 9/p35/p10 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1B7G2

Cat No : 66169-1-PBS

Print datasheet

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6

验证数据展示

  • WB analysis of Jurkat using 66169-1-Ig (same clone as 66169-1-PBS)

    WB analysis of Jurkat using 66169-1-Ig (same clone as 66169-1-PBS)

    Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/p35/p10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

    WB analysis of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

    IP experiment of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

    IP result of anti-Caspase 9/p35/p10 (IP:66169-1-Ig, 5ug; Detection:66169-1-Ig 1:500) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66169-1-Ig (same clone as 66169-1-PBS)

    IHC staining of human lymphoma using 66169-1-Ig (same clone as 66169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 66169-1-Ig (same clone as 66169-1-PBS)

    IHC staining of human lymphoma using 66169-1-Ig (same clone as 66169-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66169-1-Ig (Caspase 9/p35/p10 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

    IF Staining of HeLa using 66169-1-Ig (same clone as 66169-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 66169-1-Ig(Caspase 9/p35/p10 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66169-1-PBS in a different storage buffer formulation.

产品信息

66169-1-PBS targets Caspase 9/p35/p10 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse
Immunogen Caspase 9/p35/p10 fusion protein Ag20813 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caspase 9, apoptosis-related cysteine peptidase
Synonyms APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa, 35 kDa
GenBank Accession NumberBC002452
Gene Symbol CASP9
Gene ID (NCBI) 842
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55211
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].