Caspase 9/p35/p10 Monoclonal antibody
Caspase 9/p35/p10 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse and More (3)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B7G2
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66169-1-Ig targets Caspase 9/p35/p10 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB,IHC,IF,ELISA |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, sheep, pig |
| Immunogen | Caspase 9/p35/p10 fusion protein Ag20813 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | caspase 9, apoptosis-related cysteine peptidase |
| Synonyms | APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, Caspase-9, ICE LAP6, ICE like apoptotic protease 6, MCH6 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 46 kDa, 35 kDa |
| GenBank Accession Number | BC002452 |
| Gene Symbol | CASP9 |
| Gene ID (NCBI) | 842 |
| RRID | AB_2833257 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P55211 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| IHC protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| IF protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| IP protocol for Caspase 9/p35/p10 antibody 66169-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways. | ||
J Pineal Res Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota. | ||
Redox Biol Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma. | ||
Chem Sci Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay. | ||
J Hazard Mater Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis. | ||
