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CoraLite® Plus 488-conjugated Caspase 9/p35/p10 Monoclonal antibody

Caspase 9/p35/p10 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

FC (Intra), IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B7G2

Cat No : CL488-66169

Print datasheet

Synonyms

APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, ICE LAP6, ICE like apoptotic protease 6, MCH6

验证数据展示

  • IF Staining of HeLa using CL488-66169

    IF Staining of HeLa using CL488-66169

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 Caspase 9/p35/p10 antibody (CL488-66169, Clone: 1B7G2 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66169

    FC experiment of HepG2 using CL488-66169

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Caspase 9/p35/p10 (CL488-66169, Clone:1B7G2) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66169-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66169 targets Caspase 9/p35/p10 in IF, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra), IF
Tested Reactivity human, mouse
Immunogen Caspase 9/p35/p10 fusion protein Ag20813 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name caspase 9, apoptosis-related cysteine peptidase
Synonyms APAF 3, APAF3, Apoptotic protease Mch 6, CASP 9, CASP9, Caspase 9, Caspase 9/p35/p10, CASPASE 9c, Caspase9, ICE LAP6, ICE like apoptotic protease 6, MCH6
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa, 35 kDa
GenBank Accession NumberBC002452
Gene Symbol CASP9
Gene ID (NCBI) 842
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55211
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caspase 9/p35/p10 antibody CL488-66169Download protocol
FC protocol for CL Plus 488 Caspase 9/p35/p10 antibody CL488-66169Download protocol
Standard Protocols
Click here to view our Standard Protocols