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ChromoTek CoraLite® Plus 555-conjugated CTLA4 VHH

CL555-ctla targets CTLA4 in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat No : CL555-ctl

Synonyms

CTLA4-Nanobody, CTLA4-Single domain antibody (sdAb), CD152, Cytotoxic T-Lymphocyte-associated antigen 4, CTLA4, CTLA-4

Validation Data Gallery

  • 1X10^6 HEK CTLA4 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl). Cells were not fixed.

    1X10^6 HEK CTLA4 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl). Cells were not fixed.

  • Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) and CL750 CD3 (CL750-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.5ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

    Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) and CL750 CD3 (CL750-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.5ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) and CL405 CD3 (CL405-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were fixed and permeabilized using Transcription Factor Staining Buffer Kit (PF00011). Lymphocytes were gated.

    Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) and CL405 CD3 (CL405-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were fixed and permeabilized using Transcription Factor Staining Buffer Kit (PF00011). Lymphocytes were gated.

  • 1x10^6 PHA-treated (3d) human PBMCs were surface stained with CL405 CD3 (CL405-65151) then fixed and permeabilized and stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl). Right: 1x10^6 PHA-treated (3d) human PBMCs were fixed and permeabilized then stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were fixed and permeabilized then stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were fixed and permeabilized using Transfripction Factor Staining Buffer Kit (PF00011). Lymphocytes were gated.

    1x10^6 PHA-treated (3d) human PBMCs were surface stained with CL405 CD3 (CL405-65151) then fixed and permeabilized and stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl). Right: 1x10^6 PHA-treated (3d) human PBMCs were fixed and permeabilized then stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were fixed and permeabilized then stained with 1ug CoraLite® Plus 555 conjugated CTLA-4 VHH (CL555-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were fixed and permeabilized using Transfripction Factor Staining Buffer Kit (PF00011). Lymphocytes were gated.

  • HeLa cells transfected with human CTLA-4 were immunostained with CoraLite® Plus 555 conjugated CTLA-4 VHH (1:500, red). Nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 40x objective and post-processed. Comment: Immunostaining with CL555-ctl can be performed live or after formaldehyde fixation.

    HeLa cells transfected with human CTLA-4 were immunostained with CoraLite® Plus 555 conjugated CTLA-4 VHH (1:500, red). Nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 40x objective and post-processed. Comment: Immunostaining with CL555-ctl can be performed live or after formaldehyde fixation.

Product Information

CL555-ctla targets CTLA4 in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateCoraLite® Plus 555 Fluorescent Dye
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity22 nM
Molecular Weight14.005 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
RRIDAB_3101938
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
BackgroundCTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

Documentation

SDS
SDS-Immuno-Oncology VHHs