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ChromoTek CTLA4 VHH, recombinant binding protein for 2x Cys conjugation

Alpaca anti-CTLA4 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

Conjugation

Conjugate

Unconjugated

Cat No : ctlCys2

Synonyms

CTLA4-Nanobody, CTLA4-Single domain antibody (sdAb), CD152, Cytotoxic T-Lymphocyte-associated antigen 4, CTLA4, CTLA-4

Validation Data Gallery

  • The affinity of anti-human CTLA recombinant VHH towards human CTLA was determined using biolayer interferometry (BLI). Biotinylated, recombinant human CTLA  was immobilized on Streptavidin biosensors and assayed with 1.5 to 100 nM of FITC Plus conjugated-CTLA VHH (FITC-ctl). Fit indicates a 1:1 binding model fitted to the data.

    The affinity of anti-human CTLA recombinant VHH towards human CTLA was determined using biolayer interferometry (BLI). Biotinylated, recombinant human CTLA  was immobilized on Streptavidin biosensors and assayed with 1.5 to 100 nM of FITC Plus conjugated-CTLA VHH (FITC-ctl). Fit indicates a 1:1 binding model fitted to the data.

Product Information

Alpaca anti-CTLA4 VHH, purified recombinant binding protein. Suitable for for cysteine conjugation with thiol-reactive reagents, e.g. maleimides. Note: unconjugated VHHs are not suited for usage without prior labeling, since they contain reactive Cysteines. Shipment and storage buffers contain TCEP to keep Cysteins reduced.

ApplicationsConjugation
ReactivityHuman
ConjugateUnconjugated
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity22 nM
Molecular Weight14.005 kDa
FormLiquid
RRIDAB_3101936
Storage Buffer10 mM HEPES, 500 mM NaCl, pH 7.0, 1 mM TCEP, 0.09% sodium azide
Storage ConditionStore at -20°C
BackgroundCTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

Documentation

SDS
SDS-Immuno-Oncology VHHs