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ChromoTek CoraLite® Plus 647-conjugated PD-L1 VHH

CL647-pdlo targets PD-L1 in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-pdlo

Synonyms

PD-L1-Nanobody, PD-L1-Single domain antibody (sdAb), B7 homolog 1, B7-H1, CD274, programmed cell death 1 ligand 1, PDL1, PD-L1

Validation Data Gallery

  • 1X10^6 HEK PD-L1 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg CoraLite® Plus 647 conjugated-PD-L1 VHH (CL647-pdlo). Cells were not fixed.

    1X10^6 HEK PD-L1 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg CoraLite® Plus 647 conjugated-PD-L1 VHH (CL647-pdlo). Cells were not fixed.

  • Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated PD-L1 VHH (CL647-pdlo) and CL488 CD3 (CL488-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated PD-L1 VHH (CL647-pdlo) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated PD-L1 VHH (CL647-pdlo) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

    Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated PD-L1 VHH (CL647-pdlo) and CL488 CD3 (CL488-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated PD-L1 VHH (CL647-pdlo) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.5ug CoraLite® Plus 647 conjugated PD-L1 VHH (CL647-pdlo) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • A431 cells transfected with human PD-L1 were immunostained with CoraLite® Plus 647 conjugated-PD-L1 VHH (1:1000, magenta). Nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with CL647-pdlo can be performed live or after formaldehyde fixation.

    A431 cells transfected with human PD-L1 were immunostained with CoraLite® Plus 647 conjugated-PD-L1 VHH (1:1000, magenta). Nuclei were stained with DAPI (cyan). Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with CL647-pdlo can be performed live or after formaldehyde fixation.

Product Information

CL647-pdlo targets PD-L1 in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateCoraLite® Plus 647 Fluorescent Dye
TypeNanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight13.887 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
RRIDAB_3661836
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
BackgroundPD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis. D-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681).

Documentation

SDS
SDS-Immuno-Oncology VHHs