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验证数据展示

  • IF Staining of PC-3 using CL488-15124

    IF Staining of PC-3 using CL488-15124

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 GSTO1 antibody (CL488-15124) at dilution of 1:200.

  • FC experiment of PC-3 using CL488-15124

    FC experiment of PC-3 using CL488-15124

    1x10^6 PC-3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Gsto1 Polyclonal Antibody (CL488-15124)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated GSTO1 Polyclonal antibody

GSTO1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-15124

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat, mouse

应用

IF/ICC, FC (Intra)

别名

Glutathione S-transferase omega-1, Glutathione S-transferase omega 1-1, glutathione S transferase omega 1, EC:2.5.1.18, EC:1.8.5.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy GSTO1 Ready-To-Use IHC Kit. GSTO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15124-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-15124 targets GSTO1 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat, mouse
免疫原 GSTO1 fusion protein Ag7199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutathione S-transferase omega 1
别名 Glutathione S-transferase omega-1, Glutathione S-transferase omega 1-1, glutathione S transferase omega 1, EC:2.5.1.18, EC:1.8.5.1
计算分子量 28 kDa
观测分子量 27-30 kDa
GenBank蛋白编号BC000127
基因名称 GSTO1
Gene ID (NCBI) 9446
RRIDAB_3672622
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78417
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glutathione-S-transferase omega 1 (GSTO1) belongs to a new subfamily of GSTs and is the rate limiting enzyme for biotransformation of inorganic arsenic, environmental carcinogen. Expression of GSTO1-1 was abundant in a wide range of normal tissues, particularly liver, macrophages, glial cells, and endocrine cells. Human GSTO1 assembles as a dimer. This antibody detects both monomer (27-30 kDa) and dimer forms of GSTO1 (54-64 kDa). (PMID: 21495794)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GSTO1 antibody CL488-15124Download protocol
FC protocol for CL Plus 488 GSTO1 antibody CL488-15124Download protocol
Standard Protocols
Click here to view our Standard Protocols