• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of mouse heart using 82886-1-RR (same clone as 82886-1-PBS)

    IHC staining of mouse heart using 82886-1-RR (same clone as 82886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 82886-1-RR (IFT88 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82886-1-PBS in a different storage buffer formulation.

  • WB analysis using 82886-1-RR (same clone as 82886-1-PBS)

    WB analysis using 82886-1-RR (same clone as 82886-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82886-1-RR (IFT88 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82886-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82886-1-RR

    Affinity and Kinetic Characterization of 82886-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82886-1-RR against Human IFT88 were performed. The affinity constant is 49.8 pM.

IFT88 Recombinant antibody, PBS Only

IFT88 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82886-1-PBS

产品说明书

CloneNo. 230074F8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074F8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

82886-1-PBS targets IFT88 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IFT88 fusion protein Ag4980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intraflagellar transport 88 homolog (Chlamydomonas)
别名 TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074F8
计算分子量 94 kDa
观测分子量 88-95 kDa
GenBank蛋白编号BC030776
基因名称 IFT88
Gene ID (NCBI) 8100
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13099
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).